The Effect of Four Weeks of Aerobic Training on The Activity of Left Ventricular Catalase Enzyme And Insulin Resistance Index In Male Rats Treated With Streptozotocin And Exposed To A High-Fat Diet

Background: A high-fat diet causes hyperinsulinemia and increases insulin resistance in the body. On the other hand, the oxidative stress caused by hyperglycemia can lead to the exacerbation of hyperglycemia and increase the complications of diabetes, and cardiovascular diseases are one of the most important complications of diabetes mellitus. Therefore, the aim of this study was to investigate the effect of 4 weeks of aerobic training on catalase enzyme activity in the left ventricle and insulin resistance index in male rats treated with streptozotocin and exposed to a high-fat diet. Methods: In this experimental study, 19 male wistar rats, with an average age of 8-12 weeks and a weight of 200-250 grams, were divided into 3 groups: diabetic control (CD 8), training diabetes (TD 8) and healthy control (C 3). The exercise program included 4 weeks of aerobic exercise with an average of 60 to 75 Vo2max in an increasing manner. Before and 48 hours after the last training session, blood was taken and left ventricular tissue samples were extracted to check the HOMA-IR index and catalase activity by ELISA method. To compare the study groups, one-way analysis of variance and Scheffe's post hoc test were used in SPSS software (p≥0.05). Results: The results showed that the induction of diabetes with STZ along with a high-fat diet causes a significant decrease in catalase enzyme (P=0.001) and a significant increase in HOMA-IR index, fasting glucose and Li index (P=0.001) in rats. However, doing 4 weeks of aerobic training caused a significant increase in catalase enzyme activity (P=0.001) and a decrease in HOMA-IR index, glucose and Li index (P=0.001). Conclusion: Aerobic exercise with moderate intensity can be used as an effective non-pharmacological method in the treatment of diabetic cardiomyopathy by increasing catalase activity and reducing resistance to insulitis in diabetes.